Immunochemistry Group Infrastructure

The infrastructure of Immunochemistry Group labs includes:

• Radioiodination unit (40 m²) equipped with safety flow radioisotope fume hoods for radiolabelling with ¹²⁵I and purification of the labeled compounds through LC and HPLC
• 12-Well gamma-counters (Berthold LB2111 and και Raw data, Oakfield, Sourcerer)
• UV-VIS spectrophotometers (Shimadzu UV-160A & Perkin-Elmer Lambda35)
• Fluorometer ((PTI, USA)
• TECAN-Spark multimode microplate reader
• ELISA plate readers (Multiscan RC by Lasbsystems OY & Sirio S by SEAC Radim Group)
• Fluorescence microtiter plate reader (Accent Fluoroscan, Lasbsystems OY)
• Luminescence microtiter plate reader (EG&G Berthold, Microlumat LB96P)
• Multi-label microtiter plate reader (optical density, fluorescence, luminescence, time-resolved fluorescence) (VICTOR3, Perkin-Elmer)
• Microtiter plate washers ((Labsystems ΟΥ και Sirio S, Seak)
• Microtiter plate orbital shakers
• Epifluorescence microscope (Axioscop, Carl Zeiss) equipped with cooled CCD camera (MicroPublisher 3.3 RTV CCD camera, QImaging) and image processing software ((ImagePro Plus 4.0)
• Microarray spotter for the preparation of protein and DNA arrays (Bio-Odyssey Caligrapher, BioRad)
• Microarray scanner equipped with lasers at 545 and 650 nm (Perkin Elmer Gx microarray scanner)
• Bright and dark field optical microscope (Axioscan, Carl Zeiss) equipped with a digital camera (Sony Cybershot)
• High Performance Liquid chromatography system (HPLC) and amino-acid analyzer with UV-Vis and fluorescence detectors (Waters)
• Preparatory HPLC system with UV detector (Waters)
• HPLC system with MS/MS triple stage quandrapole detector (VARIAN)
• Electrophoresis and liquid chromatography systems (LKB)
• Capillary electrophoresis system for proteins and DNA (BioRad)
• Thermocycler for PCR (BioRad)
• Deep freezers (-80 ^oC), freezers and refrigerators, ovens and incubators
• Ovens (up to +250 ^oC) and incubators (up to +70 ^oC)
• Ultra-pure water systems
• General laboratory equipment (magnetic stirrers, pH meters, etc.)